Imaging CXCR4 Signaling with Firefly Luciferase Complementation
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چکیده
منابع مشابه
Breakthrough Technologies Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants1[C][W][OA]
The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. The previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. To facilitate pr...
متن کاملFirefly luciferase complementation imaging assay for protein-protein interactions in plants.
The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. The previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. To facilitate pr...
متن کاملFirefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants1[C][W][OA]
The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. The previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. To facilitate pr...
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Signaling pathways regulating proliferation, differentiation, and apoptosis are commonly mediated through protein-protein interactions as well as reversible phosphorylation of proteins. To facilitate the study of regulated protein-protein interactions in cells and living animals, we optimized firefly luciferase protein fragment complementation by screening incremental truncation libraries of N-...
متن کاملSplit luciferase complementation for analysis of intracellular signaling.
Bioluminescent proteins such as luciferases are unique analytical tools with high sensitivity and wide dynamic ranges. However, applicability of the proteins has remained limited to reporter gene analysis. Split luciferase complementation technique is a new powerful strategy to detect protein-protein interactions and their related intracellular signaling in living cells. This review presents a ...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2008
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac8005457